Table 4 Colony forming unit-normalized 16S r DNA copy number for the best extraction protocols (Tables 1 , 2 and 3 ) associated with three biological replicates of various Gram-positive Eubacteria

Carnobacterium maltaromaticum

High Pure

0.711

0.679

1.34

0.909

0.374

a

Agencourt

1.56

1.24

2.07

1.62

0.419

a

OmniLyse

6.20

7.65

7.23

7.03

0.746

b

CFU mL⁻¹

5.63 × 10⁸

5.34 × 10⁸

3.89 × 10⁸

±11.1%

±22.5%

±37.6%

Enterococcus faecalis

High Pure

3.05

2.14

2.76

2.65

0.465

a

Agencourt

3.28

3.75

4.12

3.72

0.421

a

OmniLyse

10.5

9.88

12.3

10.9

1.26

b

CFU mL⁻¹

7.01 × 10⁸

6.32 × 10⁸

5.90 × 10⁸

±0.240%

±7.72%

±1.71%

Lactococcus lcatis

High Pure

4.19

3.11

2.74

3.35

0.753

ab

Agencourt

1.15

1.48

1.44

1.36

0.180

a

OmniLyse

4.58

8.28

5.47

6.11

1.93

b

CFU mL⁻¹

7.58 × 10⁸

7.55 × 10⁸

8.01 × 10⁸

±9.55%

±12.5%

±9.99%

Staphylococcus aureus

High Pure

0.307

0.328

0.349

0.328

0.0210

a

Agencourt

0.568

0.707

0.649

0.641

0.0698

a

OmniLyse

30.3

44.2

53.3

42.6

11.6

b

CFU mL⁻¹

5.54 × 10⁸

4.58 × 10⁸

4.96 × 10⁸

±7.44%

±1.66%

±14.8%

Streptococcus pneumoniae

High Pure

9.95

8.55

7.14

8.55

1.41

a

Agencourt

8.77

9.39

8.62

8.93

0.408

a

OmniLyse

7.90

7.42

6.15

7.16

0.904

a

CFU mL⁻¹

3.24 × 10⁸

3.60 × 10⁸

3.92 × 10⁸

±14.6%

±0.937%

±8.37%