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Figure 1 | BMC Microbiology

Figure 1

From: Modulation of bacterial outer membrane vesicle production by envelope structure and content

Figure 1

OMV production and Lpp crosslinking changes inversely with altered PG structure. (A) Relative fold OMV production in cultures of the indicated strains grown in LB overnight at 37°C was determined by quantitating OMVs by OMPs, normalizing to OD600, and dividing by OD600-normalized OMV production in a WT culture. (B) Relative fold crosslinked Lpp in cultures of the indicated strains grown in LB to an OD600 of ~ 0.4 at 37°C was determined by immunoblotting of PG copurified Lpp, normalizing to OD600, and dividing by OD600-normalized crosslinked Lpp in a WT culture. (C) Relative fold of free Lpp in cultures of the indicated strains grown overnight in LB was determined by quantitative immunoblotting of Lpp in whole cell preparations, normalizing to OD600, and dividing by OD600-normalized Lpp in a WT culture. (D) Relative fold OMV production in cultures of the indicated strains grown in LB overnight at 37°C was determined as in part A. (E) Relative fold crosslinked Lpp in cultures of the indicated strains grown in LB to an OD600 of ~ 0.4 at 37°C was determined as in part B. Error bars indicate standard error of the mean (SEM). p values refer to comparisons with WT. *, p ≤ 0.05; n ≥ 3.

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