Figure 2

Schematic diagram for the DNA fragments obtained by genome walking. Major facilitator superfamily (MFS) permease is a membrane transporter. TetR is a tetracycline repressor protein. Phasin is a PHA granule-associated protein. The bold arrow lines indicate the DNA fragment amplified by IAN-PCR. The scissors indicate the restriction digestion site for Eco RI. For the DNA fragment I-GG18, the Eco RI digestion site could not be identified. The downstream region of the I-GG18 PHA synthase was determined via PCR amplification using a predicted primer based on the whole genome sequence (WGS) of Marinobacter hydrocarbonoclasticus strain ATCC 49840.