Rapid species-level identification of vaginal and oral lactobacilli using MALDI-TOF MS analysis and 16S rDNA sequencing

Table 4 Identification of lactobacilli isolates with MALDI-TOF MS and 16S rDNA-analysis: Oral isolates

Species	16S rDNA-analysis	MALDI-TOF-MS	Concordance [%]*
L. salivarius	3	4	75
L. gasseri	3	3	100
L. rhamnosus	3	3	100
L. paracasei**	3	3	100
L. fermentum	2	1	50
L. oris	2	2	100
L. crispatus	2	2	100
L. kalixensis	1	1	100
L. reuteri	1	1	100
L. helveticus	1	0	-
L. ultunensis***	0	1	-

* Isolates identified as same species by both methods in relation to the total number of isolates of this species identified by 16S rDNA sequencing.
**The 16S rDNA-analysis result was L. paracasei/L. casei, both species were not distinguishable from each other.
***The BioTyper log score was 1.71, reliable identification on genus level, but not on species level, repetition of the samples resulted in L. acidophilus with a log score of 1.87.

ISSN: 1471-2180