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Table 4 Identification of lactobacilli isolates with MALDI-TOF MS and 16S rDNA-analysis: Oral isolates

From: Rapid species-level identification of vaginal and oral lactobacilli using MALDI-TOF MS analysis and 16S rDNA sequencing

Species 16S rDNA-analysis MALDI-TOF-MS Concordance [%]*
L. salivarius 3 4 75
L. gasseri 3 3 100
L. rhamnosus 3 3 100
L. paracasei** 3 3 100
L. fermentum 2 1 50
L. oris 2 2 100
L. crispatus 2 2 100
L. kalixensis 1 1 100
L. reuteri 1 1 100
L. helveticus 1 0 -
L. ultunensis*** 0 1 -
  1. * Isolates identified as same species by both methods in relation to the total number of isolates of this species identified by 16S rDNA sequencing.
  2. **The 16S rDNA-analysis result was L. paracasei/L. casei, both species were not distinguishable from each other.
  3. ***The BioTyper log score was 1.71, reliable identification on genus level, but not on species level, repetition of the samples resulted in L. acidophilus with a log score of 1.87.