Figure 6
From: Plasminogen activation by staphylokinase enhances local spreading of S. aureus in skin infections
SAK-huPli complex is protected from inhibition by α 2 AP in the presence of S. aureus bacterial surfaces. S. aureus bacterial surfaces enhance SAK-mediated plasmin generation and partially protect the SAK-huPli complex from inhibition by α2AP. Plasmin generation by SAK (6.25 nM) in a mixture of murine (0.25 μM) and human (0.05 μM) plasminogen, with or without α2AP (0.125 μM) and in the absence or presence of washed SAK-negative S. aureus LS-1 EP (OD600 2.0, 15% vol/vol). Plasmin generation was quantitated by conversion of the chromogenic substrate S-2403 and assessed in a microtiter plate ELISA reader at 405 nm. Mean and SD from 3 independent experiments. *denotes P < 0.05, **P < 0.01.