Skip to main content
Figure 3 | BMC Microbiology

Figure 3

From: Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection

Figure 3

Northern blot detection of sense sRNAs complementary to tRNA. Hybridizations detected sense tRFs from tRNA Ile_cluster0, tRNA Thr_cluster1 and tRNA Arg_cluster0 in (A) wild-type (wt) isolates 3928A and R0, and in (B) wt isolate 88069 and transformant lines silenced for PiDcl1, PiAgo1, PiAgo4, PiAgo5. Approximate sizes in nucleotides are indicated to the right of each blot. The membranes were re-probed for 5S rRNA to control for equal loading of sRNAs (bottom). The signals in (B) were quantified, and values relative to the wt and normalized to 5S rRNA, are shown below each blot.

Back to article page