Table 1 Strains and plasmids used in this study
Strain or plasmid | Relevant genotype and characteristics | Reference or source |
|---|---|---|
Strains | ||
HD73 | Bt subsp. Kurstaki carrying the cry1Ac gene | Laboratory collection |
HD(PgabT-lacZ) | Bt HD73 carrying pHT-PgabT plasmid; EmR | [8] |
HD(PgabR-lacZ) | Bt HD73 carrying pHT-PgabR plasmid; EmR | [8] |
HD(PgabTDR-lacZ) | Bt HD73 carrying pHT-PgabTDR plasmid; EmR | This study |
HD(PgabTDI-lacZ) | Bt HD73 carrying pHT-PgabTDI plasmid; EmR | This study |
HD(PsigL-lacZ) | Bt HD73 carrying pHT-PsigL plasmid; EmR | This study |
ΔgabT(PgabT-lacZ) | HD(ΔgabT) carrying pHT-PgabT plasmid; EmR | [8] |
ΔgabD(PgabT-lacZ) | HD(ΔgabD) carrying pHT-PgabT plasmid; EmR | [8] |
ΔgabR(PgabT-lacZ) | HD(ΔgabR) carrying pHT-PgabT plasmid; EmR | [8] |
ΔsigL(PgabT-lacZ) | HD(ΔsigL) carrying pHT-PgabT plasmid; EmR | [8] |
DPAS(PgabT) | HD(ΔgabR) carrying pHT-PgabT and pHT1618-DPAS plasmid; EmR and TetR | This study |
CgabR(PgabT) | HD(ΔgabR) carrying pHT-PgabT and pHT1618-gabR plasmid; EmR and TetR | This study |
E. coli TG1 | Δ(lac-proAB) supE thi hsd- 5 (F’ traD36 proA + proB + lacI q lacZ ΔM15), general purpose cloning host | Laboratory collection |
E. coli ET12567 | F − dam-13::Tn9 dcm-6 hsdM hsdR recF143 zjj-202::Tn10 galK2 galT22 ara14 pacY1 xyl-5 leuB6 thi-1, for generation of unmethylated DNA | Laboratory collection |
E.coli BL21(DE3) | E.coli B, F − , dcm, ompT, hsdS(rB-mB-), gal, λ(DE3) | Laboratory collection |
BL21 (pET21b-gabR) | BL21(DE3) with pET21b-gabR plasmid | This study |
Plasmids | ||
pET21b | Expressional vector, ApR, 5.4 kb | Laboratory collection |
pHT304-18Z | Promoterless lacZ vector, EmR, ApR | Laboratory collection |
pHT1618 | E. coli-Bt shuttle, ApR, TetR | [25] |
pET21b-gabR | pET-21b containing gabR gene, ApR | This study |
pHT-PgabT | pHT304-18Z carrying promoter upstream from gabT | [8] |
pHT-PgabR | pHT304-18Z carrying promoter upstream from gabR | [8] |
pHT-PgabTDR | pHT304-18Z carrying promoter upstream from gabT without GabR binding site | This study |
pHT-PgabTDI | pHT304-18Z carrying promoter upstream from gabT without the first 21 repeat region of GabR binding site | This study |
pHT-PsigL | pHT304-18Z carrying promoter upstream from sigL | This study |
pHT1618-gabR | pHT1618 containing gabR promter and gabR gene, ApR | This study |
pHT1618-DPAS | pHT1618 containing gabR promter and PAS domain deletion fragment, ApR | This study |