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Figure 5 | BMC Microbiology

Figure 5

From: Activation of gab cluster transcription in Bacillus thuringiensis by γ-aminobutyric acid or succinic semialdehyde is mediated by the Sigma 54-dependent transcriptional activator GabR

Figure 5

Analysis of the role of Sigma 54 and GabR on gabR transcription. Panel A, Model of the gabR upstream region. The PgabR (633 bp) fragment contains the wild-type promoter, the PgabRD(580 bp) fragment of the gabR promoter without the −12/−24-like elements, and the PgabRE(253 bp) fragment is used for GabR binding. Panel B, EMSA for PgabRE after interaction with GabR. Lane 1, FAM-labeled PgabT probe; Lane 2, probe PgabT was incubated with 5.6 μM of purified GabR. Lane 3–6, the FAM-labeled PgabRE probe was incubated with increasing concentrations of purified GabR as shown at the top of the figure. Each lane contained 0.1 μg of probe. C. β-galactosidase activity assay of PgabR in wild-type strain HD73 (▲), the sigL mutant (●) and the gabR mutant (■). D. β-galactosidase activity assay of PgabRD in wild-type strain HD73 (▲), the sigL mutant (●) and the gabR mutant (■). T0 is the end of exponential phase, and Tn is n hours after T0.

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BMC Microbiology

ISSN: 1471-2180

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