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Figure 4 | BMC Microbiology

Figure 4

From: Activation of gab cluster transcription in Bacillus thuringiensis by γ-aminobutyric acid or succinic semialdehyde is mediated by the Sigma 54-dependent transcriptional activator GabR

Figure 4

Analysis of the transcriptional activation of gabT, gabR and sigL promoters. Panel A, analysis of β-galactosidase activity induced by the gabT promoter in strain HD73, the sigL mutant and the gabR mutant with or without GABA. Strain HD(PgabT-lacZ) without (■) and with (●) 1 mM GABA, strain ΔsigL(PgabT-lacZ) without (▲) and with (▼) 1 mM GABA, strain ΔgabR(PgabT-lacZ) without (◀) and with (▶) 1 mM GABA. Panel B, analysis of β-galactosidase activity induced by the gabT promoter in strain HD73, the sigL mutant and the gabR mutant with or without SSA. Strain HD(PgabT-lacZ) without (■) and with (●) 1 mM SSA, strain ΔsigL(PgabT-lacZ) without (▲) and with (▼) 1 mM SSA, strain ΔgabR(PgabT-lacZ) without (×) and with (○) 1 mM SSA. Panel C, analysis of β-galactosidase activity induced by the gabR promoter in strain HD73 with or without GABA and SSA. Strain HD(PgabR-lacZ) without GABA or SSA (■), with 1 mM GABA (●), and with 1 mM SSA (▲). Panel D, analysis of β-galactosidase activity induced by the sigL promoter in strain HD73 with or without GABA and SSA. Strain HD(PsigL-lacZ) without GABA or SSA (■), with 1 mM GABA (●), and with 1 mM SSA (▲). A0 is mid-exponential phase (OD600 = 1.0), and An is n hours after A0.

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BMC Microbiology

ISSN: 1471-2180

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