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Figure 4 | BMC Microbiology

Figure 4

From: Saccharomyces cerevisiae biofilm tolerance towards systemic antifungals depends on growth phase

Figure 4

Antifungal drugs are active against growing biofilms. (A) Quantitation of biofilm cell viability. Biofilms were grown for 4 hours and viability determined as CFUs. Biofilms were then treated with 10 times the MIC of the indicated antifungal drug for 24 hours and CFUs were measured. Shown is log change in CFUs after 24 hours treatment. n = 3, error bars are standard deviations. Statistical significance between treated and untreated samples was evaluated with Student's t-test. *P < 0.01. (B) GFP-tagged S. cerevisiae in 4-hour biofilms treated for 24 hours with the indicated antifungal agents. VOR: voriconazole (10 μg/ml), 5FC: flucytosine (80 μg/ml), CAS: caspofungin (10 μg/ml), and AmB: amphotericin B (10 μg/ml). Cells were visualized as described in the legend to Figure 1.

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