Chitinases play a role in C. albicans biofilm formation. Three C. albicans isolates with LBF and HBF were standardised to 1 × 106 cells/ml in RPMI-1640 and grown in 24 well microtitre plates at 37°C for 4 and 24 h. (A) Supernatants were retained and mixed with a chitinase substrate working solution for 30 min at 37°C. Fluorescence was measured at 360 and 450 nm and chitinase activity represented at U/OD, normalised to isolate biomass. Each isolate was measured in duplicate, on three separate occasions. (B-C) Biofilms with LBF (n = 3) and HBF (n = 3) were washed with PBS and RNA extracted using the TRIzol method, cDNA synthesised and real-time PCR used to measure the expression of CHT2 (B) and CHT3 (C). Percentage of gene expression is shown as log10 mean ± SD relative to housekeeping gene ACT1. *p < 0.05.