Figure 1

Variation in C. albicans biofilm formation and eDNA release. C. albicans isolates with LBF (n = 3) and HBF (n = 3) were grown as biofilms in 96 well flat-bottom microtitre plates at 37°C for 24 h. (A) Biofilm biomass was assessed spectrophotometrically by reading CV absorbance, data represents mean ± SD. (B) Biofilms were treated ± 256 μg/ml DNase for a further 24 h before being passed through 0.22 μM membrane filter. Biomass retained on the filters was dried overnight at 60°C and dry weight measurements taken. In addition, biofilms were stained with CV and imaged to show the disruptive effect of DNase on the biofilms, data represents mean ± SE. (C) Isolates were grown as biofilms for 4 and 24 h in the presence of the DNA binding dye SYBR® Green I. Fluorescence was measured after 4 and 24 h at Ex485/Em518. Absorbance was measured simultaneously for normalising the fluorescence data, data represents mean ± SD. Each isolate was tested in duplicate, on three independent occasions. *^#p < 0.05, **p < 0.01.