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Table 1 Bacterial strains used in this work

From: SinR is a mutational target for fine-tuning biofilm formation in laboratory-evolved strains of Bacillus subtilis

Strain name

Genotype

Source or strain construction

NCIB3610

ancestral biofilm-forming strain

Laboratory stock

PY79

domesticated laboratory strain

Laboratory stock

168

domesticated laboratory strain

Laboratory stock

DS92

ΔsinR::spec

Kearns et al. [14]

RL3852

ΔepsH::tet

Kearns et al. [14]

R1

sinR 319T>C in NCIB3610

This study

R3

sinR 265G>A in NCIB3610

This study

R4

sinR 171G>A in NCIB3610

This study

ST1

evolved strain isolated from static culture 1 on day 60

This study

SH1

evolved strain isolated from shaking culture 5 on day 60

This study

SH2

evolved strain isolated from shaking culture 3 on day 60

This study

SH3

evolved strain isolated from shaking culture 3 on day 60

This study

SH4

evolved strain isolated from shaking culture 3 on day 60

This study

ALM89

sacA::P epsA -lux in NCIB3610, CmR

This study

SLH20

sacA::P epsA -lux in ST1, CmR

This study

SLH21

sacA::P epsA -lux in SH2, CmR

This study

SLH22

sacA::P epsA -lux in SH3, CmR

This study

SLH23

sacA::P epsA -lux in SH4, CmR

This study

SLH24

sacA::P epsA -lux in DS92, CmR

This study

ALM91

sacA::P tapA -lux in NCIB3610, CmR

This study

SLH25

sacA::P tapA -lux in ST1, CmR

This study

SLH26

sacA::P tapA -lux in SH2, CmR

This study

SLH27

sacA::P tapA -lux in SH3, CmR

This study

SLH28

sacA::P tapA -lux in SH4, CmR

This study

SLH29

sacA::P tapA -lux in DS92, CmR

This study