Figure 3

60-day evolved strains show diverse colony architectures, with more extreme differences visible in LB colonies. Select colony-purified evolved strains were grown from single cells on LB or from 3 μl spots on MSgg (A). For comparison, LB and MSgg colony biofilms are included from deletion mutant strains ΔsinR::spec (DS92) and ΔepsH::tet (RL3852) (B). Scale bars for LB colonies are 1 mm and for MSgg colonies are 1 cm.