Figure 6
From: Bacillus anthracis genomic DNA enhances lethal toxin–induced cytotoxicity through TNF-α production
Treatment with BAG and pretreatment with TNF-α enhance LT-mediated cytotoxicity in a concentration-dependent manner. (A) J774A.1 cells were treated with medium alone, LT (0.5 μg/ml PA + 0.05 μg/ml LF), or LT + 0, 0.1, 1, or 10 μg/ml BAG. (B) J774A.1 cells were pre-treated with 0, 0.25, 0.5, or 1 μg/ml TNF-α for 24 h, and then LT (0.5 μg/ml PA +0.05 μg/ml LF) was added. After the 4-h incubation with LT, cell viability was determined using the MTT assay. The y axis represents the percent survival relative to control values and is given as the mean ± SD derived from three separate experiments. *P <0.05 as compared with the untreated control group.