Figure 4
From: Bacillus anthracis genomic DNA enhances lethal toxin–induced cytotoxicity through TNF-α production
MAPK pathways are important for BAG-induced TNF-α production. (A) RAW264.7 cells were treated with 10 μg/ml BAG for the indicated time periods. At the end of the stimulation periods, the cells were lysed, and cell lysates were subjected to western blot analysis to determine the intracellular levels of ERK, p38, and JNK, and their phosphorylated forms. (B) RAW264.7 cells were pretreated with the indicated concentrations of MAPK inhibitors including ERK (U0126), p38 (SB203580), or JNK (SP600125) for 1 h, followed by stimulation with 10 μg/ml BAG for an additional 24 h. Values are the mean ± SD of triplicate samples. *P <0.05 as compared with the BAG-treated group.