Figure 2
From: Bacillus anthracis genomic DNA enhances lethal toxin–induced cytotoxicity through TNF-α production
BAG augments TNF-α protein and mRNA expression in a dose- and time-dependent manner. RAW264.7 cells were stimulated (A) with various doses of BAG (0, 0.1, 1, or 10 μg/ml) for 24 h (grey bars, for protein level) or 3 h (line, for mRNA level), or (B) with 10 μg/ml BAG for 0, 3, 6, 12, or 24 h. At the end of the stimulation, total RNA was extracted or culture supernatants were collected. Real-time RT-PCR and ELISA were conducted to analyze BAG-induced TNF-α mRNA and protein levels, respectively. Values are the mean ± SD of three replicates per group. *P <0.05 as compared with the untreated control group.