Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells

Table 1 Bacterial strains and plasmids used in this study

*E. coli*strains and plasmids	Characteristics	Reference
Strains
1551-2	aEPEC (ONT:H⁻) isolated from a diarrheic child, which expresses LA on HeLa cells (Nal^R)	[18]
1551-2ΔfimA	1551-2 fimA::zeo (Nal^R Zeo^R), T1P mutant	[24]
1551-2ΔfimA(pBAD)	1551-2 mutant harboring pBAD/Myc-His A vector (Nal^R Zeo^R Amp^R)	[24]
1551-2ΔfimA(pFimA)	1551-2 mutant harboring pFimA (Nal^R Zeo^R Amp^R)	[24]
Plasmid
pBAD/Myc-His A	Cloning vector, harboring the araBAD promoter (pBAD) from E. coli, which is induced in the presence of L-arabinose.	Invitrogen
pFimA	pBAD/Myc-His A vector harboring the fimA gene from aEPEC 1551-2	[24]

Nal^R: nalidixic acid resistant; Zeo^R: zeocin resistant; Amp^R: ampicillin resistant; LA: Localized adherence.

ISSN: 1471-2180