Figure 1From: Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells Comparison of quantitative and qualitative adherence of aEPEC 1551–2 strain to HeLa cells under different environmental conditions. (A) Number of adherent bacteria recovered after 6 h of incubation with HeLa cells, demonstrating the positive influence of CO2 (5%) in the adhesiveness of aEPEC 1551–2. (B, C, D and E) Distinct adherence patterns observed in 1551–2 interaction with HeLa cells, after incubation in medium containing 5 mM (B and D) or 25 mM (C and E) glucose, in a normal air atmosphere (B and C) and in an atmosphere of 5% CO2 (D and E). The hybrid LA/AA-like pattern is observed over and around cells, only in assays performed in medium containing 25 mM glucose (C and E). * and ** represent statistical differences observed between experiments performed under 0.03% and 5% CO2 concentration, in medium containing low (P =0.03) or high (P =0.002) glucose concentration, respectively.Back to article page