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Figure 1 | BMC Microbiology

Figure 1

From: Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells

Figure 1

Comparison of quantitative and qualitative adherence of aEPEC 1551–2 strain to HeLa cells under different environmental conditions. (A) Number of adherent bacteria recovered after 6 h of incubation with HeLa cells, demonstrating the positive influence of CO2 (5%) in the adhesiveness of aEPEC 1551–2. (B, C, D and E) Distinct adherence patterns observed in 1551–2 interaction with HeLa cells, after incubation in medium containing 5 mM (B and D) or 25 mM (C and E) glucose, in a normal air atmosphere (B and C) and in an atmosphere of 5% CO2 (D and E). The hybrid LA/AA-like pattern is observed over and around cells, only in assays performed in medium containing 25 mM glucose (C and E). * and ** represent statistical differences observed between experiments performed under 0.03% and 5% CO2 concentration, in medium containing low (P =0.03) or high (P =0.002) glucose concentration, respectively.

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