Construction of a mariner-based transposon vector for use in insertion sequence mutagenesis in selected members of the Rhizobiaceae

Table 1 Summary of pSAM_Rl transposition in RLV3841

Replicon	Size (bp)	GC%	Gene number	Potential insertions	Observed insertions	Insertion density^A	Mean read count^B	Median read count^C
Chrom	5057142	61.10	4800	89660	73375	0.82	20.46	13.00
RL12	870021	61.00	790	14845	13122	0.88	18.57	12.00
RL11	684202	61.00	644	12456	10964	0.88	19.97	13.00
RL10	488135	59.60	471	10003	8470	0.85	17.19	10.00
RL9	352782	61.00	313	6359	5453	0.86	19.65	13.00
RL8	147463	58.70	142	3118	2702	0.87	17.96	11.00
RL7	151546	57.60	186	3615	2458	0.68	22.09	12.00
Genome	7751291	60.00	7346	140056	116544	0.83	19.41	12.00

^AInsertion Density is the fraction of the 'TA' insertions sites that contain a Tn insertion.
^BMean Read Count is the mean number of Tn insertions within 'TA' sites.
^CMedian Read Count is the median number of Tn insertions within a specific 'TA' site.

ISSN: 1471-2180