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Table 5 Stool samples analysis

From: Hepatitis A virus subgenotyping based on RT-qPCR assays

Stool Age/Sex Transaminases IU/mL Travel Genotype by sequencing HAV genome copies/g of stool Difference of quantification between 5’UTR and subtype RT-qPCR assays (log10) Difference of quantification between IA and IIA subtype RT-qPCR assays (log10)
5’UTR IA IB IIA IIB IIIA IIIB
0780627147 21-30/M NC none IA 2.30 x107 1.93 x108 - - - - - IA : −0.92 -
1280210015 51-60/F 5680 Senegal IB 1.50 x1010 - 7.19 x1010 - - - - IB : −0.68 -
1280514230 51-60/F 7191 Benin IB 6.85 x1010 - 6.37 x1010 - - - - IB : +0.03 -
1181216151 51-60/F 3623 none IA 7.75 x1011 5.75 x1011 - 3.85 x106 - - - IA : +0.13 ; IIA : +5.30 −5.17
078014121 51-60/F NC Morocco IB 1.12 x107 - 3.73 x105 - - - - IB : +1.48 -
  1. Each sample was tested with the reference RT-qPCR assay targeting the 5’UTR of HAV and the 6 genotype-specific RT-qPCR assays. The subtyping results were compared with those obtained with sequencing by the NRC. Concentrations are given in genome copies per gram of stool. NC = Not communicated. The difference of quantification between 5’UTR and subtype RT-qPCR assays is calculated by the formula: (log10 (genomes copies determined by reference RT-qPCR/genomes copies determined by subgenotyping RT-qPCR assays)). The difference of quantification between IA and IIA subtypes by RT-qPCR assays is calculated by the formula: (log10 (genomes copies determined by IA RT-qPCR/genomes copies determined by IIA RT-qPCR assays)).