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Table 4 Specificity of subgenotyping RT-qPCR assays

From: Hepatitis A virus subgenotyping based on RT-qPCR assays

  Set primers/probe
  HAV IA HAV IB HAV IIA HAV IIB HAV IIIA HAV IIIB
Sample HAV IA 30.01 ± 0.58 (6/6) - - - - -
HAV IB - 29.60 ± 1.15 (6/6) - - - -
HAV IIA - - 30.57 ± 0.87 (6/6) - - -
HAV IIB - - 35.02 ± 1.31 (4/6) 25.53 ± 0.35 (6/6) - -
HAV IIIA - - - - 36.90 ± 1.69 (6/6) -
HAV IIIB - - - - - 27.65 ± 0.48 (6/6)
  1. Six subtyping RT-qPCR assays were tested with 5 x 104 genome copies/assay for all subtypes of HAV in duplicate in three different experiments. Results are expressed as means cycle threshold (Ct) values ± standard deviations (SD). The number of positive Ct values is given in parentheses.