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Table 4 Specificity of subgenotyping RT-qPCR assays

From: Hepatitis A virus subgenotyping based on RT-qPCR assays

 

Set primers/probe

 

HAV IA

HAV IB

HAV IIA

HAV IIB

HAV IIIA

HAV IIIB

Sample

HAV IA

30.01 ± 0.58 (6/6)

-

-

-

-

-

HAV IB

-

29.60 ± 1.15 (6/6)

-

-

-

-

HAV IIA

-

-

30.57 ± 0.87 (6/6)

-

-

-

HAV IIB

-

-

35.02 ± 1.31 (4/6)

25.53 ± 0.35 (6/6)

-

-

HAV IIIA

-

-

-

-

36.90 ± 1.69 (6/6)

-

HAV IIIB

-

-

-

-

-

27.65 ± 0.48 (6/6)

  1. Six subtyping RT-qPCR assays were tested with 5 x 104 genome copies/assay for all subtypes of HAV in duplicate in three different experiments. Results are expressed as means cycle threshold (Ct) values ± standard deviations (SD). The number of positive Ct values is given in parentheses.
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BMC Microbiology

ISSN: 1471-2180

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