Table 3 Characteristics of RT-qPCR standard curves
From: Hepatitis A virus subgenotyping based on RT-qPCR assays
Genome copies/RT-qPCR assay | Mean Ct values +/− SD | ||||||
|---|---|---|---|---|---|---|---|
HAV IA | HAV IB | HAV IIA | HAV IIB | HAV IIIA | HAV IIIB | HAV 5'UTR | |
5 x 107 | / | / | / | / | 26,56 ± 0,89 | / | / |
5 x 106 | / | / | / | / | 29,49 ± 0,88 | / | / |
5 x 105 | 26.28 ± 0.59 | 26.54 ± 0.95 | 26.64 ± 0.60 | 22.25 ± 0.45 | 32.97 ± 0.94 | 24.50 ± 0.50 | 27.07 ± 1.00 |
5 x 104 | 30.01 ± 0.58 | 29.60 ± 0115 | 30.26 ± 0.52 | 25.53 ± 0.35 | 36.90 ± 1.69 | 27.65 ± 0.48 | 30.76 ± 0.74 |
5 x 103 | 33.34 ± 0.66 | 32.66 ± 1.47 | 33.82 ± 0.56 | 28.62 ± 0.66 | 41.34 ± 0.69 | 31.58 ± 0.47 | 33.32 ± 0.79 |
5 x 102 | 36.28 ± 0.69 | 36.16 ± 1.48 | 36.59 ± 0.79 | 32.00 ± 0.67 | nd | 35.64 ± 1.48 | 37.16 ± 1.83 |
5 x 101 | nd | nd | nd | 35.79 ± 1.09 | / | nd | nd |
5 x 100 | nd | nd | nd | nd | / | nd | nd |
E | 99.0% | 109.0% | 95.8% | 100.8% | 83.9% | 86.5% | 102.2% |
R2 | 0.974 | 0.898 | 0.973 | 0.982 | 0.952 | 0.966 | 0.916 |