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Table 3 Characteristics of RT-qPCR standard curves

From: Hepatitis A virus subgenotyping based on RT-qPCR assays

Genome copies/RT-qPCR assay Mean Ct values +/− SD
HAV IA HAV IB HAV IIA HAV IIB HAV IIIA HAV IIIB HAV 5'UTR
5 x 107 / / / / 26,56 ± 0,89 / /
5 x 106 / / / / 29,49 ± 0,88 / /
5 x 105 26.28 ± 0.59 26.54 ± 0.95 26.64 ± 0.60 22.25 ± 0.45 32.97 ± 0.94 24.50 ± 0.50 27.07 ± 1.00
5 x 104 30.01 ± 0.58 29.60 ± 0115 30.26 ± 0.52 25.53 ± 0.35 36.90 ± 1.69 27.65 ± 0.48 30.76 ± 0.74
5 x 103 33.34 ± 0.66 32.66 ± 1.47 33.82 ± 0.56 28.62 ± 0.66 41.34 ± 0.69 31.58 ± 0.47 33.32 ± 0.79
5 x 102 36.28 ± 0.69 36.16 ± 1.48 36.59 ± 0.79 32.00 ± 0.67 nd 35.64 ± 1.48 37.16 ± 1.83
5 x 101 nd nd nd 35.79 ± 1.09 / nd nd
5 x 100 nd nd nd nd / nd nd
E 99.0% 109.0% 95.8% 100.8% 83.9% 86.5% 102.2%
R2 0.974 0.898 0.973 0.982 0.952 0.966 0.916
  1. Parameters of RT-qPCR amplification curves obtained for HAV detection by the RT-qPCR reference method and HAV subgenotyping by RT-qPCR assays. The limit of detection (LOD) has been defined as the lowest amount of HAV detected in the three experiments and is shown in bold. nd: not detected. / : not analyzed.