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Figure 5 | BMC Microbiology

Figure 5

From: Requirement of HIV-1 Vif C-terminus for Vif-CBF-β interaction and assembly of CUL5-containing E3 ligase

Figure 5

Effects of Vif mutations in the region from amino acid 124-141 on the antiviral activity of A3F. (A) Effects of WT and mutant Vif proteins on A3F antiviral activity. HIV-1 viruses were produced by transfecting HEK293T cells with NL4-3ΔVif and A3F along with VR1012 as a control vector or WT or various Vif mutants as indicated. Virus infectivity was assessed using MAGI indicator cells, with the virus infectivity in the presence of WT Vif set to 100%. Error bars represent the standard deviation from triplicate wells. (B) Effects of WT and mutant Vif proteins on A3F degradation and virion packaging. A3F expression was assessed by Western blotting against A3F-V5, Vif-HA and tubulin as loading control. A3F packaging was evaluated by Western blotting against A3F-V5 and CAp24 after virus was purified from the cell culture supernatant.

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