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Table 1 Discrepancies and error in the conventional identification method (Vitek 2) and MALDI-TOF for the identification of 507 Gram-negative bacteria

From: Real-time comparative evaluation of bioMerieux VITEK MS versus Bruker Microflex MS, two matrix-assisted laser desorption-ionization time-of-flight mass spectrometry systems, for identification of clinically significant bacteria

Final identification (no of isolates) No of isolates
Conventional identification method (Vitek 2) VITEK MS identification Bruker MS identification 16S rRNA sequence result (number of discrepant organisms sequenced)
No ID Mis-ID No ID Mis - ID No ID Mis-ID  
Escherichia coli (181) 0 0 0 0 0 0  
Klebsiella pneumoniae (71) 0 0 0 0 0 1 (score <1.7) K. pneumoniae (1)
Klebsiella oxytoca (2) 0 0 0 0 0 0  
Pseudomonas aeruginosa (66) 0 0 0 0 0 0  
Acinetobacter baumannii (39) 0 0 0 0 0 1 (Acinetobacter genomospecies 13TU)*, 3 (score <1.7) A. baumannii (4)
Acinetobacter lwoffii (2) 0 0 0 0 0 1 (score <1.7) A. lwoffii (1)
Salmonella spp. (29) 0 0 0 0 0 1 (score <1.7) Salmonella spp. (1)
Enterobacter cloacae (14) 0 0 0 0 0 0  
Enterobacter aerogenes (7) 0 0 0 0 0 0  
Enterobacter asburiae (5) 0 0 0 0 0 0  
Pantoea agglomerans (1) 0 0 0 0 0 0  
Proteus mirabilis (17) 0 0 0 0 0 0  
Stenotrophomonas maltophilia (18) 0 0 0 0 0 1 (score <1.7) S. maltophilia (1)
Serratia marcescens (15) 0 0 0 0 0 0  
Citrobacter koseri (9) 0 0 0 0 0 0  
Citrobacter freundii (1) 0 0 0 0 0 0  
Morganella morganii (7) 0 0 0 0 0 0  
Achromobacter xylosoxidans (5) 0 0 0 0 0 2 (Acinetobacter spp.) A. xylosoxidans (2)
Providencia rettgeri (4) 0 2 (Providencia stuartii) 0 2 (P. stuartii) 0 1 (score <1.7) P. rettgeri (2)
Burkholderia cepacia (2) 0 0 0 0 0 1 (score <1.7) B. cepacia (1)
Sphingomonas paucimobilis (2) 0 0 0 0 0 2 (score <1.7) S. paucimobilis (2)
Aeromonas hydrophila/caviae (2) 0 0 0 0 0 2 (Aeromonas jandaei) A. hydrophila/caviae (2)
Ralstonia solanacearum (1) 0 1(Ralstonia pickettii) 0 1 (R. pickettii) 0 1 (Ralstonia insidiosa) R. solanacearum (1)
Chryseobacterium indologenes (1) 0 0 0 0 0 0  
Plesiomonas shigelloides (1) 0 0 0 0 0 0  
Vibrio parahaemolyticus (1) 0 0 0 0 0 0  
Campylobacter jejuni (1) 0 0 0 0 0 0  
Bordetella pertussis (1) 0 0 0 0 0 0  
Bordetella parapertussis (1) 0 0 0 0 0 0  
Yersinia enterocolitica (1) 0 0 0 0 0 0  
  1. No ID = no identification in spite of its presence in database; Mis-ID = misidentification; *Current name is Acinetobacter nosocomialis.