Table 1 Bacterial strains and plasmids used in this study

P. aeruginosa

  PAO1

Wild-type

[31]

  PAO1 fpvR

PAO1 with fpvR (PA2388) deletion

[10]

  PAO1 fpvR _1–89-TAP

PAO1 containing mini CTX2:: fpvR _1–89 -TAP

This study

  PAO1fpvR; pUCP23::fpvR _1–89-TAP

PAO1fpvR containing pUCP23::fpvR _1–89-TAP; Gm^R, Cb^R

This study

E. coli

  S17-1

hsd ^R hsdM ⁺ recA thi pro [integrated RP 4-2-Tc::Mu, Km::Tn7]; Sm ^R Tp ^R

[32]

  JM83

F- ara Δ (lac-proAB) rpsL [Φ80, lacZ ΔM15] thi

[33]

  MC1061

ΔlacX 74 Hsr⁻ Hsm⁺ rpsL

[34]

  MC1061 (DE3)

MC1061 lysogenized with λDE3 [lacI lac UV5-T7 gene 1]

This study

Plasmids

  pUCP23

lacI ^q lacZ(α-fragment) aacC1 ColE1 ori; RO1600 ori; Cb/Amp^R, Gm^R

[35]

  pUCP23::fpvR _1-89TAP

519 bp PCR fragment containing the fpvR promoter and 5′end of fpvR, joined to a C-terminal TAP-tag and cloned into pUCP23

This study

  Mini-CTX2

Self-proficient integration vector; Tc^R

[36]

  Mini-CTX2::fpvR _1-89TAP

519 bp PCR fragment containing the fpvR promoter and 5′end of fpvR, joined to a C-terminal TAP-tag and cloned into Mini-CTX2

This study

  pFLP2

pRO1600 ori, sacB, flp recombinase; Ap^R/Cb^R

[37]

  pMP190::P _fpvA _lacZ

fpvA promoter cloned upstream of lacZ in pMP190; Cm^R

[6]

  pMP190::P _pvdE _lacZ

pvdE promoter cloned upstream of lacZ in pMP190; Cm^R

[30],[38]

  pETDuet

Dual expression vector; Ap^R

[39]

  pETDuet::fpvR _1–89

fpvR _1–89 cloned into MCS2 of pETDuet

This study

  pETDuet::fpvI

fpvI cloned into MCS1 of pETDuet

This study

  pETDuet::pvdS

pvdS cloned into MCS1 of pETDuet

This study

  pETDuet::fpvI_fpvR _1–89

fpvI cloned into MCS1 and fpvR _1–89 cloned into MCS2 of pETDuet

This study

  PETDuet::pvdS _fpvR _1–89

pvdS cloned into MCS1 and fpvR _1–89 cloned into MCS2 of pETDuet

This study