Figure 7
The activity of FpvI and PvdS C-terminal deletion mutants. (A) An alignment of FpvI showing the 5 amino acid C-terminal deletion and PvdS showing the 13 amino acid C-terminal deletion. (B and C) β-galactosidase assays were carried out with E. coli MC1061 (DE3) containing pMP190::P fpvA _lacZ or pMP190::P pvdE _lacZ, along with pETDuet expressing FpvR1–89 and C-terminal deletion mutants of (B) FpvI or (C) PvdS. Values are compared to WT FpvI and PvdS and were obtained from three biological replicates. Error bars are ±1 SD.