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Figure 5 | BMC Microbiology

Figure 5

From: Interactions between an anti-sigma protein and two sigma factors that regulate the pyoverdine signaling pathway in Pseudomonas aeruginosa

Figure 5

The activity of FpvI and PvdS mutants in the absence of FpvR 1–89 . β-galactosidase assays were carried out with E. coli MC1061 (DE3) containing (A) pMP190::P fpvA _lacZ or (B) pMP190::P pvdE _lacZ, along with pETDuet expressing (A) mutant FpvI or (B) mutant PvdS. Dark grey bars: FpvI and PvdS mutants generated by error prone PCR. Patterned bars: FpvI and PvdS mutants engineered by site directed mutagenesis. Light grey bars: WT FpvI or PvdS. Data were obtained from three biological replicates and error bars are ±1 SD. Statistically significant difference to WT according to Student's T-test is indicated: *p <0.05, **p <0.01.

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