Purpose | Primer | Sequence |
---|---|---|
Cloning of 400 bp fragment of fucP (Bbr_1742) in pORI19 | FucPF | TAGCATAAGCTT GGCGAATCGTTCGTATCA |
 | FucPR | GATATCTCTAGA GCGCCCCAGTGCTTGAGC |
Cloning of 479 bp fragment of lnbP (Bbr_1587) in pORI19 | LnbPF | TAGCATAAGCTT CACACAGGTATTGGGAGGTTG |
 | LnbPR | CTAGTCTCTAGA GTTGTAGGCGCCACCATCC |
Cloning of 568 bp fragment of lacZ7 (Bbr_1833) in pORI19 | LacZ7F | TAGCATAAGCTT CCAGGCCAAGAACTCCAGTG |
 | LacZ7R | CATGATTCTAGA CAGCTTGGGCAGGTTGAACG |
Amplification of tetW | TetWF | TCAGCTGTCGAC ATGCTCATGTACGGTAAG |
 | TetWR | GCGACGGTCGAC CATTACCTTCTGAAACATA |
Confirmation of site-specific homologous recombination | FucPconfirm | TGTTCGCCATGTTCGTTATC |
 | LnbPconfirm | GATCACTCTGCATATGGACG |
 | LacZ7confirm | GTACCGACATCGACGCGTTC |