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Table 2 Oligonucleotide primers used in this study

From: Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium

Purpose

Primer

Sequence

Cloning of 400 bp fragment of fucP (Bbr_1742) in pORI19

FucPF

TAGCATAAGCTT GGCGAATCGTTCGTATCA

 

FucPR

GATATCTCTAGA GCGCCCCAGTGCTTGAGC

Cloning of 479 bp fragment of lnbP (Bbr_1587) in pORI19

LnbPF

TAGCATAAGCTT CACACAGGTATTGGGAGGTTG

 

LnbPR

CTAGTCTCTAGA GTTGTAGGCGCCACCATCC

Cloning of 568 bp fragment of lacZ7 (Bbr_1833) in pORI19

LacZ7F

TAGCATAAGCTT CCAGGCCAAGAACTCCAGTG

 

LacZ7R

CATGATTCTAGA CAGCTTGGGCAGGTTGAACG

Amplification of tetW

TetWF

TCAGCTGTCGAC ATGCTCATGTACGGTAAG

 

TetWR

GCGACGGTCGAC CATTACCTTCTGAAACATA

Confirmation of site-specific homologous recombination

FucPconfirm

TGTTCGCCATGTTCGTTATC

 

LnbPconfirm

GATCACTCTGCATATGGACG

 

LacZ7confirm

GTACCGACATCGACGCGTTC

  1. Restriction sites incorporated into oligonucleotide primer sequences are indicated in italics.