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Table 1 Bacterial strains and plasmids used in this study

From: Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium

Strains and plasmids

Relevant features

Reference or source

Strains

  

Escherichia coli strains

  

E.coli EC101

Cloning host; repA + kmr

[37]

E.coli EC101-pNZ-M.Bbrll + Bbr11

EC101 harboring a pNZ8048 derivative containing bbrllM and bbrlllM

[39]

Bifidobacterium sp. strains

  

B. breve UCC2003

Isolate from a nursling stool

[36]

B. breve UCC2003 - pAM5

B. breve UCC2003 harboring pAM5

This study

B. breve UCC2003-nanA

pORI19-Tet-nanA (Bbr_0168) insertion mutant of B. breve UCC2003

[13]

B. breve UCC2003-fucP

pORI19-Tet-fucP (Bbr_1742) insertion mutant of B. breve UCC2003

This study

B. breve UCC2003-lnbP

pORI19-Tet-lnbP (Bbr_1587) insertion mutant of B. breve UCC2003

This study

B. breve UCC2003-lacZ7

pORI19-Tet-lacZ7 (Bbr_1833) insertion mutant of B. breve UCC2003

This study

B. breve UCC2003-galT

Tetr transposon mutant of B. breve UCC2003

[41]

B. bifidum PRL2010

Isolate from infant faeces

[26]

B. bifidum PRL2010-pPKCM7

B. bifidum PRL2010 harboring pPKCM7

This study

Plasmids

  

pAM5

pBC1-puC19-Tcr

[38]

pPKCM7

pblueCm harboring rep pCIBA089

[40]

pORI19

Emr, repA-, ori+, cloning vector

[37]

pORI19-tetW-fucP

Internal 400 bp fragment of fucP and tetW cloned in pORI19

This study

pORI19-tetW-lnbP

Internal 479 bp fragment of lnbP and tetW cloned in pORI19

This study

pORI19-tetW-lacZ7

Internal 568 bp fragment of lacZ7 and tetW cloned in pORI19

This study