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Table 1 Bacterial strains and plasmids used in this study

From: Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium

Strains and plasmids Relevant features Reference or source
Strains   
Escherichia coli strains   
E.coli EC101 Cloning host; repA + kmr [37]
E.coli EC101-pNZ-M.Bbrll + Bbr11 EC101 harboring a pNZ8048 derivative containing bbrllM and bbrlllM [39]
Bifidobacterium sp. strains   
B. breve UCC2003 Isolate from a nursling stool [36]
B. breve UCC2003 - pAM5 B. breve UCC2003 harboring pAM5 This study
B. breve UCC2003-nanA pORI19-Tet-nanA (Bbr_0168) insertion mutant of B. breve UCC2003 [13]
B. breve UCC2003-fucP pORI19-Tet-fucP (Bbr_1742) insertion mutant of B. breve UCC2003 This study
B. breve UCC2003-lnbP pORI19-Tet-lnbP (Bbr_1587) insertion mutant of B. breve UCC2003 This study
B. breve UCC2003-lacZ7 pORI19-Tet-lacZ7 (Bbr_1833) insertion mutant of B. breve UCC2003 This study
B. breve UCC2003-galT Tetr transposon mutant of B. breve UCC2003 [41]
B. bifidum PRL2010 Isolate from infant faeces [26]
B. bifidum PRL2010-pPKCM7 B. bifidum PRL2010 harboring pPKCM7 This study
Plasmids   
pAM5 pBC1-puC19-Tcr [38]
pPKCM7 pblueCm harboring rep pCIBA089 [40]
pORI19 Emr, repA-, ori+, cloning vector [37]
pORI19-tetW-fucP Internal 400 bp fragment of fucP and tetW cloned in pORI19 This study
pORI19-tetW-lnbP Internal 479 bp fragment of lnbP and tetW cloned in pORI19 This study
pORI19-tetW-lacZ7 Internal 568 bp fragment of lacZ7 and tetW cloned in pORI19 This study