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Table 2 Transposon insertions resulting in increased expression of sigS

From: Investigating the genetic regulation of the ECF sigma factor σS in Staphylococcus aureus

Accession number

NE numbera

Tn551insertion site

Gene

Hitsb

Uniquec

DNA metabolism: DNA replication, recombination and repair

SACOL0566

NE544

Nucleoside permease

nupC

1

1

Regulators

SACOL0513

NE1883

Transcriptional regulatory protein

glcT

1

1

SACOL1451

NE1684

Response regulator

arlR

1

1

SACOL1436

NE9

Modulator of SarA

msa

1

1

SACOL2086

NE1218

Transcriptional regulator, TenA family

tenA

1

1

SACOL2188

NE436

Lactose phosphotransferase system repressor

lacR

5

1

Cell envelope associated

SACOL1472

NE1

Cell wall associated fibronectin-binding protein

ebh

6

2

Transporters

SACOL0684

NE1292

Na+/H + antiporter, MnhE component, putative

 

2

1

SACOL1319

NE1580

Glycerol uptake facilitator protein

 

5

1

SACOL1392

NE142

Sodium:alanine symporter family protein

 

3

1

SACOL1414

NE1609

Peptide ABC transporter, ATP-binding protein

 

4

1

Amino acid biosynthesis

SACOL0168

NE595

Glutamate N-acetyltransferase/amino-acid acetyltransferase

argJ

2

1

SACOL1349

NE809

Threonine aldolase

 

1

1

SACOL1448

NE1391

Dihydroipoamide succinyltransferase

sucB

1

1

SACOL1449

NE547

2-Oxoglutarate dehydrogenase, E1 component

sucA

3

1

SACOL1478

NE1136

Alanine dehydrogenase

 

1

1

SACOL2045

NE1177

Ketol-acid reductoisomerase

 

1

1

Protein synthesis and modification

SACOL1369

NE1904

50S ribosomal protein l33

 

4

1

SACOL1480

NE582

Hypothetical protein (ribosome associated GTPase domain)

 

1

1

Unknown function

SACOL1452

NE577

PAP2 family protein

 

1

1

  1. aNE numbers are the identification number in the Nebraska Transposon Mutant Library.
  2. bHits refer to the total number of Tn551 insertion sites identified for a particular gene.
  3. cThe unique number of insertions sites refers to those insertions that are a result of distinctive insertion events.