Figure 3
From: Investigating the genetic regulation of the ECF sigma factor σS in Staphylococcus aureus
Identification of positive regulators of sigS expression. Mutant strains bearing a sigS-lacZ fusion were grown in TSB at 37°C and sampled after 5 h of growth. β-Galactosidase activity was measured using 4-MUG as a substrate to determine sigS expression levels. Assays were performed on duplicate samples and the values averaged. The results presented are from three independent experiments. Error bars are shown as ± SEM. Significance was determined using a Student t test; *indicates a p value of <0.05.