Figure 2
From: Investigating the genetic regulation of the ECF sigma factor σS in Staphylococcus aureus
Transcriptional profiling of sigS in transposon mutants found to negatively effect expression. (A, B, and C) Mutant strains bearing a sigS-lacZ fusion were grown in TSB at 37°C and samples withdrawn at the times specified. β-Galactosidase activity was measured using 4-MUG as a substrate to determine sigS expression levels. Assays were performed on duplicate samples and the values averaged. The results presented are from three independent experiments. Error bars are shown as ± SEM. Significance was determined by a Student t test; *indicates a p value of <0.05.