Figure 7
From: Activation of epidermal growth factor receptor is required for Chlamydia trachomatis development
EGFR is required for the reorganization of F-actin at the periphery of chlamydial inclusions. (A-C) Organization of F-actin at the chlamydial inclusion periphery. HeLa cells treated with (A) control siRNA, (B) EGFR siRNA or (C) Erlotinib, were infected with C. trachomatis for 24 h, fixed, and processed for confocal microscopy. F-actin was detected with Alexa Fluor 488-phalloidin (green) and chlamydial inclusions were detected using anti-chlamydial LPS antibody (red). Note the distinct assembly of F-actin at the inclusion periphery in (A) which is altered upon inhibition of EGFR (B and C). (D) HeLa cells treated with IgG or Cetuximab were infected with Ct for 24 h, fixed, and processed for confocal microscopy. F-actin was detected with Alexa Fluor 488-phalloidin (green) and chlamydial inclusions were detected using anti-chlamydial LPS antibody (red). Note the distinct assembly of F-actin at the inclusion periphery in IgG treated cells and the lack of it in Cetuximab treated cells. Scale bar - 10 μm. (E) Organization of F-actin at the chlamydial inclusion periphery in MEFs EGFR+/+ and MEFs EGFR-/-. MEFs EGFR+/+ and MEFs EGFR-/- were infected and processed as in (A). F-actin is noticeably rearranged at the chlamydial inclusion periphery in the MEFs EGFR+/+ but not in the MEFs EGFR-/- cells.