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Figure 3 | BMC Microbiology

Figure 3

From: Activation of epidermal growth factor receptor is required for Chlamydia trachomatis development

Figure 3

C. trachomatis activates EGFR downstream signaling. (A) Monolayers of MEFs EGFR+/+ and (C) MEFs EGFR-/- cells with and without chlamydial infection were lysed at 2.5, 5, 10 and 18 hpi. Cell lysates were immunoblotted with antibodies against phosphorylated and total EGFR, PLCγ1, Akt and STAT5. β-actin was used as loading control. Each phosphorylated protein was first normalized against the total protein and then the fold increase from °Ct to + Ct was calculated from three independent experiments (B). An increased phosphorylation of EGFR, PLCγ1, Akt and STAT5 was observed in MEFs EGFR+/+ (P < 0.05) while no change was observed in the MEFs EGFR-/- upon C. trachomatis infection.

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