Figure 6

The involvement of NOD1 in P. aeruginosa -induced hBD-2 in SW480 cells. SW480 cells were transfected with control siRNA and NOD1 siRNA (siRNA = non-target control siRNA; siNOD1 = siRNA to NOD1) for 48 hours. The transfected cells were left uninfected or infected by P. aeruginosa PAO1 for 7 hours. (A) Western blots probed with antibodies against NOD1 and GAPDH confirm knockdown of NOD1. (B) Supernatant was analyzed by ELISA for secreted IL-8 and hBD-2 protein in SW480 cells. The amount of IL-8 or hBD-2 produced is shown as the fold increase over control cells. (C) After infection, total RNA was extracted from the cells and analyzed byReal-time PCR, as described in Materials and Methods. Results are represented as means ± S.E.M. for at least three determinations from independent experiments. An asterisk indicates a significant difference (p <0.05).