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Figure 2 | BMC Microbiology

Figure 2

From: Differential regulation of interleukin-8 and human beta-defensin 2 in Pseudomonas aeruginosa-infected intestinal epithelial cells

Figure 2

The effect of prolonged P. aeruginosa infection on IL-8 and hBD-2 mRNA expression in SW480 cells. SW480 cells were left uninfected (CON) infected with the wild-type P. aeruginosa strain PAO1 for one to seven hours. Total RNA was prepared after infection and analyzed by real-time quantitative PCR to estimate amounts of IL-8 and hBD-2 transcript. The amount of IL-8 and hBD-2 mRNA produced, normalized to the corresponding amount of GAPDH transcript, is shown as the fold increase over uninfected control cells. Results are represented as means ± S.E.M. for at least three determinations from independent experiments. (*p <0.05; # p <0.05).

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