Identification of virulence associated loci in the emerging broad host range plant pathogen Pseudomonas fuscovaginae

Table 2 Virulence screening of Tn 5 transposon mutants of P. fuscovaginae in C. quinoa plants

*Pseudomonas fuscovaginae(Pfv*)strains	Lesion scores in screen I(Average ± S.D.)	Lesion scores in screen II(Average ± S.D.)
Pfv UPB0736 (WT)	5, 5, 5 (5 ± 0)	5, 5, 5, 5, 5, 5 (5 ± 0)
Pfv 80	0, 1, 1 (0.66 ± 0.58)^a	2, 2, 2, 2, 2, 3 (2.16 ± 0.40)^a
Pfv 90	1, 1, 1 (1 ± 0)^a	0, 0, 0, 0, 0, 1 (0.16 ± 0.40)^a
Pfv 102	0, 0, 0 (0 ± 0)^a	0, 1, 1, 2, 2, 3 (1.50 ± 1.05)^a
Pfv 169	0, 0, 0 (0 ± 0)^a	0, 0, 0, 0, 0, 0 (0 ± 0)^a
Pfv 188	0, 0, 0 (0 ± 0)^a	0, 0, 0, 0, 0, 0 (0 ± 0)^a
Pfv 270	1, 1, 1 (1 ± 0)^a	1, 1, 1, 1, 1, 1 (1 ± 0)^a
Pfv 420	2, 2, 3 (2.33 ± 0.58)^a	2, 2, 3, 3, 3, 3 (2.66 ± 0.52)^a
Pfv 445	1, 1, 1 (1 ± 0)^a	2, 2, 2, 2, 2, 2 (2 ± 0)^a
Pfv 480	1, 1, 1 (1 ± 0)^a	1, 1, 1, 1, 1, 1 (1 ± 0)^a

Table showing disease severity for wild type and selected Tn5 mutants of Pfv based on their rating score in C. quinoa plants. A two-tailed, paired `t’ test with 95% of confidence intervals for independent means was performed between the wild type and each of Tn5 mutants. ^a; significant difference to WT at P <0.05.

ISSN: 1471-2180