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Table 1 Bacterial strains used in this study

From: Identification of virulence associated loci in the emerging broad host range plant pathogen Pseudomonas fuscovaginae

Strains

Relevant characteristicsa

Reference/source

E. coli.

  

DH5a

Cloning strain, Nalr

[35]

PRK2013

Helper strain for tri-parental conjugation, Kmr

[36]

Pseudomonas fuscovaginae (Pfv)

  

Pfv UPB0736 (NCPPB 3871) (WT)

Wild-type strain isolated from diseased rice in Madagascar; Nfr, Ampr

[34]

Pfv 80

80:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 90

90:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 102

102:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 169

169:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 188

188:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 270

270:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 420

420:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 445

445:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 480

480:: Tn5 of Pfv UPB0736; Nfr, Kmr

This study

Pfv 80-pKNOCK

80:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 90-pKNOCK

90:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 102-pKNOCK

102:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 169-pKNOCK

169:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 188-pKNOCK

188:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 270-pKNOCK

270:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 420-pKNOCK

420:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 445-pKNOCK

445:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 480-pKNOCK

480:: pKNOCK mutant of Pfv UPB0736; Nfr, Kmr

This study

Pfv 90 + pCos90

pCos90:: Pfv 90; Nfr, Kmr,Tcr

This study

Pfv 420 + pCos420

pCos420:: Pfv 420; Nfr, Kmr,Tcr

This study

Pfv 445 + pCos445

pCos445:: Pfv 445; Nfr, Kmr,Tcr

This study

  1. a;Nalr, Nfr Kmr, Tcr and Ampr indicates nalidixic acid, nitrofurantoin, kanamycin, tetracycline and ampicillin respectively.