Figure 4

CP1 blocks CH-1a viral particle release. (A and B) Cells were infected with CH-1a at an MOI of 0.01 at 37°C for 24 h, and then treated with different concentrations of CP1 or YPD (control) for 2 or 4 h. The cells and supernatants were harvested to quantify the intracellular (A) and extracellular (B) viral particles. (C) Viral titers in the supernatants were measured after CP1 (480 μg/ml) treatment for 2 or 4 h. Data are the results of three independent experiments (means ± SE). Significant differences compared with the YPD-treated control group are denoted: *P <0.05, **P <0.01, or ***P <0.001.