Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection of novel avian influenza A (H7N9) virus

Table 2 Comparison of detection of H7N9 virus by using RT-LAMP and rRT-PCR assay

	Strain	Virus Titer (PFU/reaction)	rRT-PCR(H7)(Ct)	rRT-PCR (N9) (Ct)	RT-LAMP (H7) (Tp,min)	RT-LAMP (N9) (Tp, min)
H7N9	A/Anhui/1/2013	200	26.11	25.89	17.9	21.1
		20	29.35	29.71	20.4	23.5
		2	33.24	32.16	25	27.6
		0.2	ND^a	>35	28.9	37.8
		0.02	ND	ND	ND	ND
		0.002	ND	ND	ND	ND

ISSN: 1471-2180