Figure 3
Fold change in the abundance of proteins related to uptake and catabolism of benzoate (1ab), 4-methylbenzoate (2ab) and succinate (3abc) during anaerobic growth of Magnetospirillum sp. strain pMbN1 with substrate mixtures or single substrates (M, 4-methylbenzoate; B, benzoate; S, succinate; the adaptation substrate is listed first in each case). Sampling points for proteomic analyses are marked in green in the growth curves displayed on the left panel. Identified proteins are marked in blue. For further details (including abundance profiles of Had-1 and Had-2) see Additional file 1: Table S1. *, Transcript profiles of selected genes during growth with the ternary substrate mixture are shown in Figure 2. Protein names (in alphabetical order): (AcnB) aconitase 2, (BclA) benzoate-CoA ligase, (BcrCBAD) benzoyl-CoA reductase, (BenK) benzoate/H+ symporter, (Dch-1) cyclohex-1,5-diene-1-carbonyl-CoA hydratase, (Dch-2) 4-methylcyclohex-1,5-diene-1-carbonyl-CoA hydratase, (DctA) C4-dicarboxylate/Na+ symporter, (DctPQM) TRAP-type C4-dicarboxylate uptake transporter, (FumA) fumarate hydratase, (GltA) citrate synthase, (Had-1) 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase, (Had-2) 6-hydroxy-4-methylcyclohex-1-ene-1-carbonyl-CoA dehydrogenase, (Icd) isocitrate dehydrogenase, (KorAB) 2-oxoglutarate:ferredoxin oxidoreductase, (MaeB) NADP+-dependent malic enzyme, (MbrCBAD) 4-methylbenzoyl-CoA reductase, (MclA) 4-methylbenzoate-CoA ligase, (Mdh) malate dehydrogenase, (Oah-1) 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, (Oah-2) 4-methyl-6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, (Orf870-960) predicted ABC-type 4-methylbenzoate uptake transporter, (PdhABC, LpdA) pyruvate dehydrogenase complex, (SdhABCD) succinate dehydrogenase, (SucAB, Lpd) 2-oxoglutarate dehydrogenase complex, (SucDC) succinyl-CoA ligase. Compound names: 1, benzoate; 2, benzoyl-CoA; 3, cyclohex-1.5-diene-carbonyl-CoA; 4, 4-methylbenzoate; 5, 4-methylbenzoyl-CoA; 6, 4-methylcyclohex-1.5-diene-1-carbonyl-CoA; 7, succinate.