Figure 6

H-NS-His protein binds to the ygeH regulatory region. Panel A: EMSA using a 228 bp fragment corresponding to the ygeH operon promoter region (nucleotides -207 to +21) and H-NS-His protein. 1) No protein, 2-8) increasing concentrations of H-NS-His (0.1, 0.2, 0.5, 1, 1.5, 2 and 4 μM, respectively). Panel B: Competitive EMSA using the DNA fragments corresponding to the ygeH operon promoter region and an excess of 3 fold of the competitor DNA (hlyR), in the presence of increasing concentrations of H-NS. 1) No protein, 2-5) increasing concentrations of H-NS-His (1, 1.5, 2 and 4 μM, respectively).