Table 2 List of primers used in this study
From: senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence
Primer | Sequence (53) | Purpose of amplification |
|---|---|---|
dr001-F | CCCTCTAGAACGAAATCGTCGGACTGAAC | senX3-regX3 operon and flanking sequence |
dr002-R | CCCTCTAGAAGGTGAGATCGCCAAGCTAC | |
senX3-F | CCGAGTTGATCGAGCTATCC | senX3 probe and gene expression |
senX3-R | AGTGCGGTAACCAGCAGAGT | |
dl15-F | GCTGACGACTACGTGACCAA | regX3 probe |
dl16-R | CGACGAGCAGCTTTGTCC | |
kan-F | CTGTGCTCGACGTTGTCACT | kanamycin gene |
kan-R | AGCCAACGCTATGTCCTGAT | |
hyg4-F | AGGTCTTCCCGGAACTGCTG | hygromycin gene |
hyg4-R | TCCTCGAACACCTCGAAGTC | |
regX3-F | TGTTGATTGTGGAGGACGAG | gene expression |
regX3-R | CGCAACTGCTTGCATACATC | |
pstC2-F | GTCGATCATCTTCGGGTTGT | gene expression |
pstC2-R | ATTTGGATCAGCGGAGTCTG |