Figure 1
From: senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence
Genetic confirmation of complementation of senX3 ::Tn and regX3 ::Tn. (A). Diagram of a 2824-bp fragment containing the senX3-regX3 operon from the genome of wild-type CDC1551. (B) FseI digestion of genomic DNA and hybridization with dig-labeled senX3 probe reveals fragments measuring 3.7-kb and 5.7-kb in CDC1551 and senX 3::Tn, respectively, as well as 5.7-kb and 7.7-kb fragments in senX3::Tn Comp. (C) SphI digestion of genomic DNA followed by hybridization with dig-labeled regX3 probe reveals 5.5-kb and 7.5-kb fragments in CDC1551 and regX3::Tn, respectively, as well as 7.5-kb and 4.6-kb fragments in regX3::Tn Comp. (D) Southern analysis of senX3 complementation. Lanes 1 and 2: SenX3::Tn Comp candidates with appropriately-sized bands; Lane 3: SenX 3::Tn; Lane 4: Wild-type CDC1551. (E) Southern analysis of regX3 complementation. Lane 1: Wild-type CDC551; Lane 2: regX 3::Tn; Lanes 35: regX3::Tn Comp candidates with appropriately-sized bands.