Skip to main content
Figure 4 | BMC Microbiology

Figure 4

From: A naturally occurring nucleotide polymorphism in the orf2/folc promoter is associated with Streptococcus suis virulence

Figure 4

Constructs used in this study. V[10] depicts the clone that was identified using a complementation strategy containing two incomplete ORFs (greyish blue arrows) and a putative operon (purple) containing orf2[10] and folC[10] preceded by the putative promoter of the operon (for clarification only the sequence of the -35 region (TGGACA) of the putative promoter is depicted in the diagram). Constructs were made that contained either orf2[10] or folC[10] (purple) preceded by the putative -35 region of the putative promoter region of the operon (purple). A construct containing orf2 from strain S735 (green) with the -35 region of the putative S735 promoter (TGGTCA) (green) was made. The same construct was mutagenized to contain the -35 region of the putative promoter sequence of strain 10 (TGGACA) (purple) yielding orf2[735][t488a].

Back to article page