Figure 1

Innate immune response of porcine PBMCs to S. suis isolates. Porcine PBMCs were incubated with S. suis strains S735-pCOM1 and S735-pCOM1-orf2[10] at an MOI of 1. Gene expression of IL-1-β (panel A), IL-6 (panel B), IL-8 (panel C), IL-10 (panel D), TNF-α (panel E), IFN-γ (panel F), and IL-12 (panel G) was determined using qPCR after 2h (white bars), 4h (hatched bars) and 6h (black bars) of stimulation. Relative expression was determined by expressing the amount of target gene relative to a housekeeping gene. LPS: lipopolysaccharide; PBS: phosphate buffered saline. Each bar represents two individual experiments each performed in duplo. Error bars represent standard error of the mean. Significance was determined by 2-way ANOVA analysis, only significant differences between PBS treatment, S735-pCOM1 and S735-pCOM1-orf2[10] are indicated, *p <0.05; **p <0.01; ***p <0.001.