Figure 4

The Haloferax volcanii THI4 homolog is required for growth in the absence of thiamine and the thiazole moiety 4-methyl-5-(β-hydroxyethyl)thiazole (THZ). Hfx. volcanii strains including H26 parent (wt), HM1052 (ΔubaA), NC1011 (Δthi4), NC1011-pJAM202c (Δthi4 + empty vector), NC1011-pJAM2821 (Δthi4 + HvThi4), and NC1011-pJAM2822 (Δthi4 + HvThi4 C165A) were grown in GMM supplemented with and without thiamine or THZ as indicated and described in the Materials and Methods. First round (A) and successive growth curves (B and C) are presented with the mean ± standard deviations shown.