Skip to main content
Figure 3 | BMC Microbiology

Figure 3

From: Conserved active site cysteine residue of archaeal THI4 homolog is essential for thiamine biosynthesis in Haloferax volcanii

Figure 3

The Haloferax volcanii thi4 gene and its in-frame deletion. (A) Schematic representation of the thi4 gene on the genome of Hfx. volcanii DS2. HVO_0662 encodes a ThiN homolog with a predicted N-terminal helix-turn-helix (HTH) DNA binding domain. The PCR primer pairs (P1/P2 and P3/P4) used to generate the thi4 gene deletion and the PCR primer pairs (P5/P6 and P7/P8) used to screen for the thi4 gene deletion are indicated. (B) PCR products generated for the Δthi4 (Δhvo_0665) mutant and parent (H26, wt) strains using primer pairs P5/P6 and P7/P8 as indicated. Size reduction with primer pair P7/P8 and the absence of a signal with primer pair P5/P6 confirm the deletion in the Δthi4 strain.

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us