Deep sequencing of HetR-bound DNA reveals novel HetR targets in Anabaenasp. strain PCC7120

BMC Microbiology

Table 4 Strains and plasmids used in this study

Strain	Description	Source or reference
AM1359	E. coli DH10B carrying pRL623 and pRL443 for biparental conjugations, Tc^r Cm^r Em^r Ap^r	[12]
AMC1078	Anabaena sp. strain PCC7120 Wild Type	Lab strain
UHM103	Anabaena PCC7120 ΔhetR (our strain designation AMC1537)	[28]
Plasmid	Description	Source or reference
pAM505	Shuttle plasmid for expression in Anabaena, Km^r Nm^r	[12]
pAM1956	Promoterless gfpmut2 in pAM505, Km^r Nm^r	[12]
pAM4375	pAM505 carrying C-terminal 6xHis tagged HetR, Km^r Nm^r	[11]
pAM4653	pAM1956 carrying the promoter of asr1469 driving GFP, Km^r Nm^r	This work
pAM4654	pAM1956 carrying the truncated promoter of asr1469 driving GFP, Km^r Nm^r	This work
pAM4659	pAM1956 carrying the promoter of asl2028 driving GFP, Km^r Nm^r	This work
pAM4660	pAM1956 carrying the truncated promoter of asl2028 driving GFP, Km^r Nm^r	This work
pAM4662	pAM1956 carrying the promoter of alr2242 driving GFP, Km^r Nm^r	This work
pAM4695	pAM1956 carrying the promoter of alr3758 driving GFP, Km^r Nm^r	This work
pAM4696	pAM1956 carrying the truncated promoter of alr3758 driving GFP, Km^r Nm^r	This work

ISSN: 1471-2180